Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Test Name/Synonym:

Antithrombin Activity
(Synonym AT3, AT3ACT, Anti-thrombin III, Anti-thrombin 3, ATACT)

Ordering Alert:

Use the instructions on this page when Antithrombin is the only test required. If testing is for Hereditary Thrombophilia Investigations refer to Hereditary Thrombophilia Investigations

Collection Alert:

All Hospitals (Inpatients and Outpatient collection labs) can collect. For community Patient Service Centres (PSC), testing can ONLY be collected at the PSCs listed below.

Shipping and Handling Alert:

Specimens are time sensitive.

LABID (Connect Care):

LAB311

Specimen Type:

Blood

Primary Container:

LT BLUE CITRATE

Minimum Collection Volume:

Adults: One 2.7 mL LT blue citrate tube, allow the vacuum tube to completely fill, minimum fill is to frosted line on tube.
Pediatric and Neonate: One 2.7 mL or 1.8 mL LT blue citrate tube, allow the vacuum tube to completely fill, minimum fill is to frosted line on tube.

Specimen Collection Requirements:

It is important to fill tube to maximum draw.
Mix well immediately after collection by inverting tube 3 to 4 times.
Must be collected by venipuncture. If venous access not available, sample can be drawn from a line but 6x the volume of the line or 5 mL (whichever is less) must be removed prior to collection.
When using a winged blood collection set (butterfly needle), a discard tube must be used to remove the air in the tubing, if only a citrate tube is drawn or is the first tube drawn.

All Hospitals (Inpatients and Outpatient collection labs) can collect. For community Patient Service Centres (PSC), testing can ONLY be collected at the following PSCs:
Edmonton Zone:	Meadowlark Patient Service Centre Northgate Patient Service Centre Heritage Patient Service Centre Tawa Patient Service Centre Windermere Patient Service Centre Leduc Patient Service Centre Athabasca Place (Sherwood Park) Patient Service Centre
Central Zone:	Camrose Patient Service Centre Red Deer Downtown Patient Service Centre Red Deer Bower Plaza Patient Service Centre Lloydminster Patient Service Centre
North Zone:	Franklin (Fort McMurray) Patient Service Centre Grande Prairie Patient Service Centre
Calgary Zone:	All Calgary Zone Patient Service Centres
South	Medicine Hat Patient Service Centre Lethbridge Centre Village Patient Service Centre Lethbridge Highlands Patient Service Centre Brooks Patient Service Centre
Mobile	Test cannot be collected by Mobile Collection Service (MCS).

Test Resources:

Indicate test under Additional Tests Section

General Laboratory Requisition – 21302

Stability and Storage:

Room temperature – 3.5 hours

Processing:

For testing sent to Calgary:	For testing sent to Edmonton:
For specimens that will be received beyond stability: Centrifuge to obtain platelet poor plasma (platelet count <10 x 10⁹/L) Aliquot plasma as per Special Coagulation Aliquoting sent to Calgary PX06-01.600	For specimens that will be received beyond stability: Centrifuge to obtain platelet poor plasma (platelet count <10 x 10⁹/L) Aliquot plasma, using a plastic pipette, into two polypropylene aliquot tubes and freeze.

Platelet poor plasma must be prepared in accordance with the College of Physician and Surgeons of Alberta (CPSA) Laboratory Standard H.4.3.4. This must include procedures for regular verification that centrifuge(s) reliably produce plasma containing <10 x 10⁹/L residual platelets. Please contact Special Coagulation in Calgary 403-770-3598 (ext 3) or in Edmonton 780-407-8487 for detailed instructions.

Transportation:

Room temperature – uncentrifuged
Frozen (dry ice if available) - Specimens must maintain frozen state for entire duration of transport.

Testing Schedule:

Daily

Calgary:
For stat testing contact Special Coagulation Laboratory. After hours contact the Hematopathologist on call.
Edmonton:
Stat testing is available at performing sites.

Reference Interval:

Greater than or equal to 0.80 U/mL

Critical Value:

Less than 0.30 U/mL

Testing Area:

Calgary: Special Coagulation
Edmonton: Hematology

Performing Site:

Calgary	Diagnostic and Scientific Centre
Edmonton	Royal Alex Hospital and University of Alberta Hospital

Additional Test Information:

1-877-868-6848

Last Updated On:

Tuesday, March 18, 2025