Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

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Test Name/Synonym:

Direct Antiglobulin Test (DAT, Direct Coombs)

Clinical Indications:

The DAT is useful in the diagnosis of autoimmune hemolytic anemia (AIHA), hemolytic disease of the newborn (HDN), red blood cell sensitization caused by drugs, and Investigation of transfusion reactions.

For suspected transfusion reactions, see Transfusion Reaction Investigation

Test Includes:

Patients greater than or equal to 4 months:
Polyspecific and/or monospecific direct antiglobulin test (anti-human globulin and anti-complement testing).
If polyspecific test is positive, monospecific testing with anti-IgG and anti-complement reagents is performed.

Neonates (less than 4 months):
Monospecific anti-IgG testing only

Collection Alert:

Avoid hemolysis

LABID (Connect Care):

LAB41779

Test Code:

CALGARY ZONE:	DAT, DATN (NEONATE)
CENTRAL ZONE:	DAT, DATNB (NEONATE)
NORTH ZONE:	DAT, DATRO (NON-TESTING SITES)
SOUTH ZONE:	DAT

Specimen Type:

Blood

Specimen Source:

Venous, Arterial, Capillary

Primary Container:

Lavender Top - EDTA

Alternate Containers:

Calgary Zone:	Microcollection EDTA
Central Zone:	Microcollection EDTA
Edmonton Zone:	N/A
North Zone:	N/A
South Zone:	Microcollection EDTA

Minimum Collection Volume:

1 mL
250 μL (Microcollection – neonates only)

Specimen Collection Requirements:

For specimen collection and testing for DAT on cord blood, see Neonatal Evaluation (Cord)

For specimen collection and testing for suspected transfusion reactions, see Transfusion Reaction Investigation

Stability and Storage:

Refrigerated 1-6⁰C and retain a minimum of 7 days

Processing:

Do not centrifuge

Method and Interpretation of Results:

A positive DAT result may indicate autoimmune hemolytic anemia, hemolytic transfusion reaction, hemolytic disease of the newborn or drug interactions. Clinical correlation and consultation is recommended if the patient’s clinical condition is suggestive of hemolysis.

Patients with a positive DAT require serological crossmatch regardless of antibody screen result.

Routine Turn Around Time:

Stat – performed within 90 minutes for ED patients, or 120 minutes for hospital patients if ordered at a testing site and required for transfusion.

Testing Schedule:

Daily

Reference Interval:

Negative

Testing Area:

Transfusion Medicine

Performing Site:

Calgary Zone	Foothills Medical Centre
Central Zone	Camrose HC* Drumheller HC* Red Deer Regional Hospital Centre Wainwright HC* Wetaskiwin Hospital*
Edmonton Zone	Grey Nuns Hospital Misericordia Hospital Royal Alex Hospital Sturgeon Hospital University of Alberta Hospital
North Zone	Bonnyville HC Cold Lake HC Edson HC Grande Prairie Regional Hospital High Prairie HC* Hinton HC Lac La Biche HC Peace River HC* St. Paul HC
South Zone	Chinook Regional Hospital Medicine Hat Regional Hospital

*polyspecific testing only. Monospecific referred out

Last Updated On:

Tuesday, August 11, 2020